東　剣虹    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast.
Journal	Formal name：Molecular genetics and genomics : MGG Abbreviation：Mol Genet Genomics ISSN code：(1617-4623)1617-4623(Linking)
Volume, Issue, Page	292(2),pp.475-481
Author and coauthor	Shimasaki Takafumi, Ohtsuka Hokuto, Naito Chikako, Azuma Kenko, Tenno Takeshi, Hiroaki Hidekazu, Murakami Hiroshi, Aiba Hirofumi
Publication date	2017/04
Summary	Overexpression of Ecl1-family genes (ecl1 +, ecl2 +, and ecl3 +) results in the extension of the chronological life span in Schizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein. Utilizing ICP-AES (inductively coupled plasma atomic emission spectroscopy) analysis, we found that wild-type Ecl1 proteins contain zinc, while cysteine-mutated Ecl1 proteins do not. We also analyzed the effect of environmental zinc on the chronological life span. We found that zinc limitation extends the chronological life span, and this extension depends on the Ecl1-family proteins.
DOI	10.1007/s00438-016-1285-x
PMID	28160081