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Luna Izuhara
Department School of Medicine, School of Medicine Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. |
| Journal | Formal name：PloS one Abbreviation：PLoS One ISSN code：(1932-6203)1932-6203(Linking) |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 10(2),pp.e0117682 |
| Author and coauthor | Izuhara Luna†, Tatsumi Norifumi, Miyagawa Shuji, Iwai Satomi, Watanabe Masahito, Yamanaka Shuichiro, Katsuoka Yuichi, Nagashima Hiroshi, Okano Hirotaka J, Yokoo Takashi |
| Authorship | Lead author |
| Publication date | 2015/02 |
| Summary | Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future. |
| DOI | 10.1371/journal.pone.0117682 |
| PMID | 25671605 |