イズハラ ルナ   Luna Izuhara
  伊豆原 るな
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor.
掲載誌名 正式名:PloS one
略  称:PLoS One
ISSNコード:(1932-6203)1932-6203(Linking)
掲載区分国外
巻・号・頁 10(2),e0117682頁
著者・共著者 Izuhara Luna†, Tatsumi Norifumi, Miyagawa Shuji, Iwai Satomi, Watanabe Masahito, Yamanaka Shuichiro, Katsuoka Yuichi, Nagashima Hiroshi, Okano Hirotaka J, Yokoo Takashi
発行年月 2015/02
概要 Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future.
DOI 10.1371/journal.pone.0117682
PMID 25671605