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大澤 重仁
Department Research Institutes and Facilities, Research Institutes and Facilities Position |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Accelerated polymer–polymer click conjugation by freeze–thaw treatment |
| Journal | Formal name：Bioconjugate chemistry Abbreviation：Bioconjug Chem ISSN code：10431802/15204812 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 23(8),pp.1503-1506 |
| Total page number | 4 |
| Author and coauthor | Hiroyasu Takemoto, Kanjiro Miyata, Takehiko Ishii, Shota Hattori, Shigehito Osawa, Nobuhiro Nishiyama, Kazunori Kataoka |
| Publication date | 2012/08/15 |
| Summary | Herein, we report a unique technique to accelerate polymer–SNA conjugation based on copper-free click chemistry: gradual freeze–thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature. |
| DOI | 10.1021/bc300182y |