オオサワ シゲヒト   Oosawa Shigehito
  大澤 重仁
   所属   医学研究科 医学研究科 (医学部医学科をご参照ください)
   職種   非常勤講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Accelerated polymer–polymer click conjugation by freeze–thaw treatment
掲載誌名 正式名:Bioconjugate chemistry
略  称:Bioconjug Chem
ISSNコード:10431802/15204812
掲載区分国外
巻・号・頁 23(8),pp.1503-1506
総ページ数 4
著者・共著者 Hiroyasu Takemoto, Kanjiro Miyata, Takehiko Ishii, Shota Hattori, Shigehito Osawa, Nobuhiro Nishiyama, Kazunori Kataoka
発行年月 2012/08/15
概要 Herein, we report a unique technique to accelerate polymer–SNA conjugation based on copper-free click chemistry: gradual freeze–thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.
DOI 10.1021/bc300182y