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論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Accelerated polymer–polymer click conjugation by freeze–thaw treatment
掲載誌名	正式名：Bioconjugate chemistry 略　 称：Bioconjug Chem ISSNコード：10431802/15204812
掲載区分	国外
巻・号・頁	23(8),pp.1503-1506
総ページ数	4
著者・共著者	Hiroyasu Takemoto, Kanjiro Miyata, Takehiko Ishii, Shota Hattori, Shigehito Osawa, Nobuhiro Nishiyama, Kazunori Kataoka
発行年月	2012/08/15
概要	Herein, we report a unique technique to accelerate polymer–SNA conjugation based on copper-free click chemistry: gradual freeze–thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.
DOI	10.1021/bc300182y