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大澤 重仁
Department Research Institutes and Facilities, Research Institutes and Facilities Position |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Enhanced function of chondrocytes in a chitosan‐based hydrogel to regenerate cartilage tissues by accelerating degradability of the hydrogel via a hydrolysable crosslinker |
| Journal | Formal name：Journal of applied polymer science. ISSN code：00218995/10974628 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 137(19),pp.1-8 |
| Total page number | 8 |
| Author and coauthor | Shohei Ishikawa, Kazutoshi Iijima, Daisuke Matsukuma, Michihiro Iijima, Shigehito Osawa, Hidenori Otsuka |
| Authorship | Corresponding author |
| Publication date | 2020/05/15 |
| Summary | Chitosan-based hydrogels as scaffolds for culturing chondrocytes were prepared using linkers with and without hydrolysable poly(DL-lactide) (PLA) segments. The evaluation of the cultured chondrocytes in them indicated that the accelerated degradation of the hydrogel via hydrolysis of the PLA slightly promoted production of the sulfated glycosaminoglycan and drastically improved that of collagen from the encapsulated chondrocytes, which are the chondrospecific extracellular matrix components. Furthermore, the accelerated degradability significantly upregulated the gene expression for Collagen II production and downregulated that for Collagen I production of the encapsulated chondrocytes. Because major component of the articular cartilage tissue is Collagen II-rich hyaline cartilage, these results suggest the degradation of the scaffolds is an important parameter in cartilage tissue regeneration and the accelerated degradability may have benefits on promotion of cartilage tissue regeneration especially from the viewpoint of hyaline cartilage-like collagen production. |
| DOI | 10.1002/app.48893 |