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論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Direct Observation of Cell Surface Sialylation by Atomic Force Microscopy Employing Boronic Acid–Sialic Acid Reversible Interaction
掲載誌名	正式名：Analytical chemistry 略　 称：Anal Chem ISSNコード：00032700/15206882
掲載区分	国外
巻・号・頁	92(17),pp.11714-11720
著者・共著者	Shigehito Osawa, Akira Matsumoto, Yukie Maejima, Toshihiro Suzuki, Yuji Miyahara, Hidenori Otsuka
担当区分	筆頭著者
発行年月	2020/07/29
概要	Tracing cell surface sialylation dynamics at a scale of the glycolipoprotein microdomain (lipid rafts) formations remains an intriguing challenge of cellular biology. Here, we demonstrate that this goal is accessible, taking advantage of a boronic acid (BA)-based reversible molecular recognition chemistry. A BA-end-functionalized poly(ethylene glycol) was decorated onto an atomic force microscopy (AFM) cantilever, which provided a dynamic and sialic acid (SA)-specific imaging mode. Using this technique, we were able to heat map the SA expression levels not only on protein-decorated substrates but also directly on the cell surfaces, with a submicrometer scale resolution that may be relevant to that of the lipid rafts formation. The SA specificity and the binding reversibility of the probe were confirmed from its pH-dependent characteristics and an inhibition assay using free state SA. This finding may provide a noninvasive means for assessing a variety of SA-involved glycosylation dynamics spanning from physiology to pathology.
DOI	https://doi.org/10.1021/acs.analchem.0c01705