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大澤 重仁
Department Research Institutes and Facilities, Research Institutes and Facilities Position |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode |
| Journal | Formal name：Bioconjugate chemistry Abbreviation：Bioconjug Chem ISSN code：10431802/15204812 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 32(2),pp.239-244 |
| Total page number | 5 |
| Author and coauthor | Akira Matsumoto, Shigehito Osawa, Takahiro Arai, Yukie Maejima, Hidenori Otsuka, Yuji Miyahara |
| Authorship | 2nd author |
| Publication date | 2021/01/22 |
| Summary | Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation. Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states. A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was “grafted-to” a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide–alkyne cycloaddition reaction. Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen. Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production. |
| DOI | 10.1021/acs.bioconjchem.0c00657 |