Department   Other, Other
Article types Original article
Language English
Peer review Peer reviewed
Title Production of scaffold-free cell-based meat using cell sheet technology.
Journal Formal name:NPJ science of food
Abbreviation:NPJ Sci Food
ISSN code:23968370/23968370
Domestic / ForeginForegin
Volume, Issue, Page 6(1),pp.41
Author and coauthor TANAKA Ryu-Ichiro†, SAKAGUCHI Katsuhisa*, YOSHIDA Azumi, TAKAHASHI Hironobu, HARAGUCHI Yuji, SHIMIZU Tatsuya*
Publication date 2022/09
Summary In the production of cell-based meat, it is desirable to reduce animal-derived materials as much as possible to meet the challenges of sustainability. Here, we demonstrate the "cell sheet-based meat": scaffold-free cell-based meat using cell sheet technology and characterize its texture and nutrients. Bovine myoblast cell sheets were prepared using temperature-responsive culture dishes (TRCDs) and 10 stacked cell sheets to fabricate three-dimensional tissue of 1.3-2.7 mm thickness. Hardness was increased by incubation on the TRCD and was further increased by boiling as is characteristic of natural meat. The wet weight percentage of total protein in the cell sheet was about half that of beef. In this method, large-sized items of cell sheet-based meat were also created by simply scaling up the TRCD. This method promises an environment-friendly food product.
DOI 10.1038/s41538-022-00155-1
PMID 36057641