マルヤマ　タクマ   丸山　拓真    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	P2X2 receptors supply extracellular choline as a substrate for acetylcholine synthesis.
掲載誌名	正式名：FEBS open bio 略　 称：FEBS Open Bio ISSNコード：22115463/22115463
巻・号・頁	12(1),pp.250-257
著者・共著者	Maruyama Takuma, Mano Asuka, Ishii Toshiyuki, Kakinuma Yoshihiko, Kaneda Makoto
発行年月	2022/01
概要	Acetylcholine (ACh), an excitatory neurotransmitter, is biosynthesized from choline in cholinergic neurons. Import from the extracellular space to the intracellular environment through the high-affinity choline transporter is currently regarded to be the only source of choline for ACh synthesis. We recently demonstrated that the P2X2 receptor, through which large cations permeate, functions as an alternative pathway for choline transport in the mouse retina. In the present study, we investigated whether choline entering cells through P2X2 receptors is used for ACh synthesis using a recombinant system. When P2X2 receptors expressed on HEK293 cell lines were stimulated with ATP, intracellular ACh concentrations increased. These results suggest that P2X2 receptors function in a novel pathway that supplies choline for ACh synthesis.
DOI	10.1002/2211-5463.13332
PMID	34787962