マルヤマ　タクマ   丸山　拓真    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Involvement of the C-terminal domain in cell surface localization and G-protein coupling of mGluR6.
掲載誌名	正式名：Journal of neurochemistry 略　 称：J Neurochem ISSNコード：14714159/00223042
巻・号・頁	158(4),pp.837-848
著者・共著者	Rai Dilip, Akagi Takumi, Shimohata Atsushi, Ishii Toshiyuki, Gangi Mie, Maruyama Takuma, Wada-Kiyama Yuko, Ogiwara Ikuo, Kaneda Makoto
発行年月	2021/08
概要	Metabotropic glutamate receptor 6, mGluR6, interacts with scaffold proteins and Gβγ subunits via its intracellular C-terminal domain (CTD). The mGluR6 pathway is critically involved in the retinal processing of visual signals. We herein investigated whether the CTD (residues 840-871) was necessary for mGluR6 cell surface localization and G-protein coupling using mGluR6-CTD mutants with immunocytochemistry, surface biotinylation assays, and electrophysiological approaches. We used 293T cells and primary hippocampal neurons as model systems. We examined C-terminally truncated mGluR6 and showed that the removal of up to residue 858 did not affect surface localization or glutamate-induced G-protein-mediated responses, whereas a 15-amino acid deletion (Δ857-871) impaired these functions. However, a 21-amino acid deletion (Δ851-871) restored surface localization and glutamate-dependent responses, which were again attenuated when the entire CTD was removed. The sequence alignment of group III mGluRs showed conserved amino acids resembling an ER retention motif in the CTD. These results suggest that the intracellular CTD is required for the cell surface transportation and receptor function of mGluR6, whereas it may contain regulatory elements for intracellular trafficking and signaling.
DOI	10.1111/jnc.15217
PMID	33067823