コバヤシ　ジユン   KOBAYASHI Jiyun   小林　純    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Flexible and facile coating of visible light-crosslinkable poly(N-isopropylacrylamide) for preparation of thermoresponsive cell culture surfaces
掲載誌名	正式名：RSC Applied Polymers 略　 称：RSC Appl Polym ISSNコード：2755-371X
掲載区分	国外
巻・号・頁	pp.in press
著者・共著者	KOBAYASHI Jun†*, OKANO Teruo
担当区分	筆頭著者,責任著者
発行年月	2026/07
概要	This paper describes the development of a visible-light-crosslinkable thermoresponsive polymer for fabricating cell culture substrates that enable temperature-dependent alteration of cell attachment and detachment. Poly(N-isopropylacrylamide) (PNIPAAm) copolymer with camphorquinone (CQ) side chains (PNIPAAm-CQ) was synthesized to enable covalent immobilization onto polystyrene surfaces via visible-light-induced radical reactions. Two coating strategies—spin-coating and adsorption—were used to fabricate thermoresponsive surfaces. Both coating methods achieved comparable polymer grafting densities; however, they exhibited distinct surface properties. Spin-coated surfaces exhibited pronounced temperature-dependent changes in receding contact angles and fibronectin adsorption, resulting in efficient endothelial cell attachment at 37 °C and rapid detachment upon lowering the temperature to 20 °C. In contrast, surfaces prepared by the adsorption method showed reduced molecular reorientation of grafted PNIPAAm chains, lower protein adsorption, and slower cell detachment kinetics. Confluent endothelial cell sheets could be harvested from both surface types by reducing the temperature from 37 °C to 20 °C while preserving cell–cell junctions. Notably, spin-coated surfaces enabled efficient cell sheet detachment, whereas adsorption-based coating has the potential to be applied for non-planar substrates. Eventually, this study demonstrates a facile and flexible coating approach for fabricating thermoresponsive culture substrates using visible-light-reactive CQ, highlighting the influence of coating methodology on surface behavior and cell interactions. These findings advance cell sheet engineering for regenerative medicine applications.
DOI	10.1039/D6LP00147E