キクチ　テツタロウ   KIKUCHI Tetsutarou   菊地　鉄太郎    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	A contractile force measurement system for hiPSC-derived cardiac tissue integrated with an ultrathin, stretchable nanomesh.
掲載誌名	正式名：Lab on a chip 略　 称：Lab Chip ISSNコード：14730189/14730189
掲載区分	国外
巻・号・頁	pp.Online ahead of print
著者・共著者	IWAI Shogo†, SASAKI Daisuke, KIKUCHI Tetsutaro*, MATSUURA Katsuhisa, FUKUDA Kenjiro, Sunghoon Lee*, SHIMIZU Tatsuya*, SOMEYA Takao, UMEZU Shinjiro*
担当区分	責任著者
発行年月	2026/05
概要	Mechanical stretch is a vital factor influencing the structural and functional properties of cardiac tissue, including its contractile force. While various platforms using elastic substrates have been developed to measure the contractile force under mechanical stretching in real time, significant challenges remain, such as achieving mechanical stability, maintaining sustained cell adhesion, and ensuring seamless system integration. In this study, we developed a contractile force measurement system for hiPSC-derived cardiac tissue using an ultrathin, stretchable polyurethane nanomesh substrate that addresses these limitations. The porous, fibrous structure of the nanomesh provides high mechanical compliance and stretchability, effectively serving as a biomimetic elastic substrate for cardiac tissue. Furthermore, the structure facilitates mechanical interlocking with the tissue, demonstrating significantly more sustained cell adhesion than does a flat substrate. Using this system, we successfully demonstrated stretch-dependent increases in contractile force, a phenomenon central to the Frank-Starling mechanism, and significant stretch-dependent differences in pharmacological responses. Our results suggest that this system could serve as a valuable platform for investigating cardiac function and pharmacological responses under mechanical stretch in vitro.
DOI	10.1039/d6lc00182c
PMID	42065540