ヨシダ　アズミ   YOSHIDA Azumi   吉田　杏美    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Conditioning of microalgae-derived extract nutrients for expansion of bovine myogenic cells in cultivated meat production
掲載誌名	正式名：Future Foods ISSNコード：2666-8335
掲載区分	国外
巻・号・頁	13,pp.101022
著者・共著者	TAKAHASHI Hironobu†*, YAMANAKA Kumiko, YOSHIDA Azumi, SUZUKI Tomoko, SHIMIZU Tatsuya
発行年月	2026/06
概要	Cultivated meat production, utilizing cell culture technology, holds the potential to alleviate the ethical, environmental, and public health concerns associated with conventional livestock-based meat production. In the new meat production, more grain cultivation will be required to produce culture medium, as glucose, amino acids, and vitamins are typically extracted from grains. Our group has previously reported that nutrients can be extracted from Chlorella vulgaris via ultrasonic fragmentation, and that the microalgae-derived medium can serve as a new basal medium for culturing bovine myogenic cells. This study reports a method to enhance the usability of Chlorella vulgaris extract-based medium by preserving it at 4 °C for 1 month. The “conditioning” process increased nutrient levels and decreased “inhibitory compounds” levels through the digestion of proteins, peptides, and polysaccharides. Consequently, the nutrient-rich medium derived from microalgae was available as a basal medium for large-scale cell expansion. In addition, the “conditioning” process effectively eliminates lot-to-lot variation in the extract, and the quality is maintained over several months. This simple protocol will facilitate the constant production of cultivated meat using a nutrient-rich, eco-friendly medium in the near future.
DOI	10.1016/j.fufo.2026.101022