ヨシダ　ケイタ   YOSHIDA Keita   吉田　慶太    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Dual roles of EGO-1 and RRF-1 in regulating germline exo-RNAi efficiency in Caenorhabditis elegans.
掲載誌名	正式名：EMBO reports 略　 称：EMBO Rep ISSNコード：14693178/1469221X
掲載区分	国外
巻・号・頁	26(18),pp.4503-4531
著者・共著者	Katsufumi Dejima, Keita Yoshida, Shohei Mitani
担当区分	2nd著者
発行年月	2025/09
概要	RNA interference (RNAi) is widely used in life science research and is critical for diverse biological processes, such as germline development and antiviral defense. In the germline of Caenorhabditis elegans, exogenous RNAi (exo-RNAi), the RNA-dependent RNA polymerases EGO-1 and RRF-1 play redundant roles in facilitating small RNA amplification. However, their coordination during the regulation of exo-RNAi processes in the germline remains unclear. Here, we examine non-null mutants of the ego-1 gene and find that ego-1(S1198L) animals exhibit germline exo-RNAi defects with normal fertility, abnormalities in germ granules, and synthetic temperature-dependent sterility with rrf-1. The exo-RNAi defects in ego-1(S1198L) are partially restored by inhibiting hrde-1 and znfx-1. Germline exo-RNAi defects are observed in wild-type and ego-1(S1198L) heterozygous descendants derived from ego-1(S1198L), but these are suppressed by ancestral inhibition of rrf-1. Our data reveal a dual role for EGO-1 in the positive regulation of germline exo-RNAi: it not only mediates target silencing through its RNA-dependent RNA polymerase activity, but also licenses exo-RNAi gene expression, which is antagonized by RRF-1.
DOI	10.1038/s44319-025-00543-0
PMID	40789965