キクチ　テツタロウ   KIKUCHI Tetsutarou   菊地　鉄太郎    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Laser-patterned microgroove polystyrene culture dishes for engineering 3D raised texture cell sheets.
掲載誌名	正式名：Biofabrication 略　 称：Biofabrication ISSNコード：17585090/17585082
掲載区分	国外
巻・号・頁	18(1),pp.015029
著者・共著者	Yue Zhang, Ziying Guo, Jie Feng, Shunfen Huang, Lin Jiang, Zhilei Zhang, Kikuchi Tetsutaro, Chengyong Wang, Botao Gao
発行年月	2026/02
概要	Techniques for fabricating cell sheets with two-dimensional (2D) patterns have advanced significantly over the years. However, creating cell sheets featuring three-dimensional (3D) raised textures that mimic the complex architecture of natural tissues continues to present a considerable challenge. This study introduces a versatile methodology for fabricating cell sheets with both 2D patterns and 3D microstructures, utilizing picosecond laser-induced microgroove-patterned polystyrene (PS) culture dishes. This technology leverages the sequential interaction of the laser with the photosensitive coating and the PS substrate to create microgrooves with exceptional precision in both width and depth. Both direct ablation and coating-assisted ablation result in patterned culture dishes that demonstrate excellent cell compatibility, an absence of cytotoxicity, and the ability to regulate cell proliferation. The patterned PS dishes create distinct 3D microenvironments that guide cell contact and adhesion arrangements, thereby modulating gene expression and protein secretion in normal human dermal fibroblasts. Notably, key proteins such as type I alpha1 collagen (Col-1), type VI alpha1 collagen (Col-6), Elastin, fibronectin, and matrix metalloproteinase 2 (MMP-2) are significantly influenced by the structure of pattern. Furthermore, cell sheets with raised textures (CSRTs) can be detached from the patterned PS culture dishes while preserving their 3D structure for over 72 h, with structural longevity dependent on feature size. To our knowledge, this study represents the first successful fabrication of CSRT using a laser-induced micro-patterning technique. This approach provides foundational insights into the development of biomimetic tissues for regenerative medicine and advancedin vitromodels, offering a promising platform for future applications in tissue engineering and biomedical research.
DOI	10.1088/1758-5090/ae2d9b
PMID	41401520