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オカザキ ケン
OKAZAKI Ken
岡崎 賢 所属 医学部 医学科（東京女子医科大学病院） 職種 教授・基幹分野長 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Bone marrow-derived fibroblast migration via periostin causes irreversible arthrogenic contracture after joint immobilization. |
| 掲載誌名 | 正式名：FASEB journal : official publication of the Federation of American Societies for Experimental Biology 略 称：FASEB J ISSNコード：15306860/08926638 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 37(5),pp.e22842 |
| 著者・共著者 | Hirotaka Iura, Kazu Kobayakawa, Hirokazu Saiwai, Daijiro Konno, Masatake Tanaka, Kazuhiro Hata, Tetsuya Tamaru, Yohei Haruta, Gentaro Ono, Kazuki Kitade, Ken Kijima, Kensuke Kubota, Yutaka Inagaki, Masato Ohtsuka, Ken Okazaki, Koji Murakami, Shusaku Matsuda, Masami Tokunaga, Takaaki Yoshimoto, Takeshi Maeda, Yasuharu Nakashima, Seiji Okada |
| 担当区分 | 最終著者 |
| 発行年月 | 2023/05 |
| 概要 | Joint contracture causes distressing permanent mobility disorder due to trauma, arthritis, and aging, with no effective treatment available. A principal and irreversible cause of joint contracture has been regarded as the development of joint capsule fibrosis. However, the molecular mechanisms underlying contracture remain unclear. We established a mouse model of knee joint contracture, revealing that fibrosis in joint capsules causes irreversible contracture. RNA-sequencing of contracture capsules demonstrated a marked enrichment of the genes involved in the extracellular region, particularly periostin (Postn). Three-dimensional magnetic resonance imaging and immunohistological analysis of contracture patients revealed posterior joint capsule thickening with abundant type I collagen (Col1a2) and POSTN in humans. Col1a2-GFPTG ; Postn-/- mice and chimeric mice with Col1a2-GFPTG ; tdTomatoTG bone marrow showed fibrosis in joint capsules caused by bone marrow-derived fibroblasts, and POSTN promoted the migration of bone marrow-derived fibroblasts, contributing to fibrosis and contracture. Conversely, POSTN-neutralizing antibody attenuated contracture exacerbation. Our findings identified POSTN as a key inducer of fibroblast migration that exacerbates capsule fibrosis, providing a potential therapeutic strategy for joint contracture. |
| DOI | 10.1096/fj.202201598R |
| PMID | 37000501 |