シミズ　タツヤ   SHIMIZU Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cardiomyocyte sheet stacking using fibrin enables high-speed construction of three-dimensional myocardial tissue and high transplantation efficiency.
掲載誌名	正式名：Biofabrication 略　 称：Biofabrication ISSNコード：17585090/17585082
掲載区分	国外
巻・号・頁	17(3),pp.035004
著者・共著者	Katsuhisa Sakaguchi, Kazuki Nakazono, Kodai Tahara, Yuto Hinata, Yusuke Tobe, Jun Homma, Hidekazu Sekine, Katsuhisa Matsuura, Kiyotaka Iwasaki, Satoshi Tsuneda, Tatsuya Shimizu
担当区分	最終著者
発行年月	2025/06/01
概要	Despite the development of three-dimensional (3D) tissues that promises remarkable advances in myocardial therapies and pharmaceutical research, vascularization is required for the repair of damaged hearts using cardiac tissue engineering. In this study, we developed a method for rapid generation of a 3D cardiac tissue, with extremely high engraftment efficiency, by stacking cardiomyocyte sheets using fibrin as an adhesive. Cell sheets were created by peeling off confluent cultured cells from a culture dish grafted with a polymer that induced surface hydrophilicity in response to low temperatures. The high engraftment rate was attributed to the retention of the adhesive protein. The multistacked vascularized cell sheets prepared using fibrin, when transplanted into the subcutaneous tissue and at myocardial infarction site in rats, yielded a transplanted 3D myocardial tissue. Furthermore, multilayered cardiomyocyte sheets were transplanted twice at 1 week intervals to create a 3D myocardial tissue. Our data suggest that fibrin-based rapidly layered cell sheets can advance tissue-engineered transplantation therapy and should aid the development of next-generation tissue-engineered products in the fields of regenerative medicine and drug screening.
DOI	10.1088/1758-5090/adcb6e
PMID	40209743