ニシダ　タカシ   NISHIDA Takashi   西田　隆司    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Identification of novel Legionella genes required for endosymbiosis in Paramecium based on comparative genome analysis with Holospora spp.
掲載誌名	正式名：FEMS microbiology ecology 略　 称：FEMS Microbiol Ecol ISSNコード：15746941/01686496
掲載区分	国外
巻・号・頁	94(11),pp.fiy162
著者・共著者	Kenta Watanabe, Haruo Suzuki, Takashi Nishida, Manami Mishima, Masato Tachibana, Masahiro Fujishima, Takashi Shimizu, Masahisa Watarai
発行年月	2018/11
概要	The relationship between Legionella and protist hosts has a huge impact when considering the infectious risk in humans because it facilitates the long-term replication and survival of Legionella in the environment. The ciliate Paramecium is considered to be a protist host for Legionella in natural environments, but the details of their endosymbiosis are largely unknown. In this study, we determined candidate Legionella pneumophila genes that are likely to be involved in the establishment of endosymbiosis in Paramecium caudatum by comparing the genomes of Legionella spp. and Holospora spp. that are obligate endosymbiotic bacteria in Paramecium spp. Among the candidate genes, each single deletion mutant for five genes (lpg0492, lpg0522, lpg0523, lpg2141 and lpg2398) failed to establish endosymbiosis in P. caudatum despite showing intracellular growth in human macrophages. The mutants exhibited no characteristic changes in terms of their morphology, multiplication rate or capacity for modulating the phagosomes in which they were contained, but their resistance to lysozyme decreased significantly. This study provides insights into novel factors required by L. pneumophila for endosymbiosis in P. caudatum, and suggests that endosymbiotic organisms within conspecific hosts may have shared genes related to effective endosymbiosis establishment.
DOI	10.1093/femsec/fiy162
PMID	30124811