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ナカヤマ マサミチ
NAKAYAMA Masamichi
中山 正道 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 講師 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Visible-light-triggered recovery of biologically intact cells using smart fluoropolymer-nanocoated materials. |
| 掲載誌名 | 正式名：Journal of controlled release : official journal of the Controlled Release Society 略 称：J Control Release ISSNコード：18734995/01683659 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 382,pp.113653 |
| 著者・共著者 | NAKAYAMA Masamichi†*, KANNO Tomonori, KIKUCHI Akihiko, TANAKA Yukiko, ANADA Takahisa, TANAKA Masaru, OKANO Teruo |
| 担当区分 | 筆頭著者,責任著者 |
| 発行年月 | 2025/03 |
| 概要 | Smart biointerfaces have attracted significant interest for regulating interactions with cells and biomacromolecules. Although stimuli-responsive changes in hydrophobicity are promising for this purpose, the effects of hydrophobic enhancement on cell adhesion behavior remain poorly understood. This study investigated a unique cell recovery system involving a visible (Vis)-light-induced change in the hydrophobicity of a smart surface from moderate to strong. To construct smart surfaces, photoresponsive spirobenzopyran-pendant fluoroalkyl polymers were spin-coated on glass coverslips. The surface properties were characterized after irradiation with ultraviolet (UV; 352 nm) or Vis (530 nm) light. Upon alternating exposure to UV and Vis light, the water wettabilities of the 1.0 w/v% polymer-coated surfaces changed (contact angles of 78° and 88°, respectively) owing to photoisomerization between the polar merocyanine and nonpolar spiropyran forms of the installed spirobenzopyrans, consistent with the observed optical properties. Atomic force microscopy showed that the polymer-coated surfaces were nanoscale flat forms without any phase-separated structures, regardless of photoswitching. After UV irradiation, bovine carotid artery endothelial cells adhered and proliferated on the moderately hydrophobic merocyanine-containing fluoropolymer surfaces. However, subsequent Vis irradiation induced spontaneous cell detachment, possibly because of the increase in surface hydrophobicity. Moreover, Vis irradiation of confluent cultured cells produced biologically intact cell sheets that retained a cell-adhesive fibronectin matrix and cell-cell junctions. This noncytotoxic Vis-triggered cell recovery system can contribute to the development of tissue engineering and cell transplantation therapies. |
| DOI | 10.1016/j.jconrel.2025.113653 |
| PMID | 40120693 |