ホンマ　ジユン   HONMA Jiyun   本間　順    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure.
掲載誌名	正式名：Communications biology 略　 称：Commun Biol ISSNコード：23993642/23993642
掲載区分	国外
巻・号・頁	8(1),pp.201
著者・共著者	KATSUURA Misako†, HOMMA Jiyun*, SEKINE Hidekazu*, SHIMIZU Tatsuya
担当区分	責任著者
発行年月	2025/02/08
概要	Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine.
DOI	10.1038/s42003-025-07627-6
PMID	39922952