シミズ　タツヤ   SHIMIZU Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure.
掲載誌名	正式名：Communications biology 略　 称：Commun Biol ISSNコード：23993642/23993642
掲載区分	国外
巻・号・頁	8(1),pp.201
著者・共著者	KATSUURA Misako†, HOMMA Jiyun*, SEKINE Hidekazu*, SHIMIZU Tatsuya
発行年月	2025/02/08
概要	Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine.
DOI	10.1038/s42003-025-07627-6
PMID	39922952