マキハラ　ヒロコ   MAKIHARA Hiroko   槇原　弘子    所属   医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	mRNA expression levels of cytochrome P450 CYP1A2, CYP3A4, and CYP3A5 in the epidermis: a focus on individual differences among Japanese individuals.
掲載誌名	正式名：Xenobiotica 略　 称：Xenobiotica ISSNコード：13665928/00498254
掲載区分	国外
巻・号・頁	54(5),pp.226-232
著者・共著者	Makihara Hiroko†, Maezawa Mika, Kaiga Kazusa, Satake Toshihiko, Muto Mayu, Tsunoda Yui, Shimada Tsutomu, Akase Tomoko*
担当区分	筆頭著者
発行年月	2024/05
概要	Various cytochrome P450 enzymes (CYPs) that contribute to drug metabolism are expressed in the skin. However, variation among individuals in CYP expression profiles is not well-understood.To investigate CYPs related to the metabolism of transdermal preparations in Japan, multiple skin tissue specimens of individuals of Japanese descent were prepared, and the mRNA expression levels of CYP1A2, CYP3A4, and CYP3A5 were measured. Associations between the expression patterns of these CYPs and body mass index (BMI) were also investigated.There were considerable individual differences in epidermal CYP1A2 mRNA expression levels, and CYP1A2 showed a weak positive correlation with CYP3A4 mRNA expression levels. In contrast to previous results for other organs, epidermal CYP3A4 mRNA expression levels showed a weak positive correlation with BMI.CYP3A4 in the epidermis may have been locally enhanced as a defence mechanism against xenobiotics in response to impaired barrier function. These differences in mRNA expression in the skin may affect the transdermal absorption of drugs, such as lidocaine and fentanyl, which are metabolised by multiple overlapping CYPs.Our study provides new insights into drug metabolism in the skin. These results are valuable for predicting drug effects and transdermal drug transfer rates in Japanese patients.
DOI	10.1080/00498254.2024.2344664
PMID	38646717