キタハラ　シュウジ   KITAHARA Shuji   北原　秀治    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   特任准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Soluble PD-L1 as a diagnostic and prognostic biomarker in resectable gastric cancer patients.
掲載誌名	正式名：Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 略　 称：Gastric Cancer ISSNコード：14363305/14363291
掲載区分	国外
巻・号・頁	26(6),pp.934-946
国際共著	国際共著
著者・共著者	Chivu-Economescu Mihaela, Herlea Vlad, Dima Simona, Sorop Andrei, Pechianu Catalin, Procop Alexandru, Kitahara Shuji, Necula Laura, Matei Lilia, Dragu Denisa, Neagu Ana-Iulia, Bleotu Coralia, Diaconu Carmen C, Popescu Irinel, Duda Dan G
発行年月	2023/11
概要	BACKGROUND:In this study, we compared programmed death-ligand 1 (PD-L1) expression in primary tissue samples and its soluble form (sPD-L1) concentration in matched preoperative plasma samples from gastric cancer patients to understand the relationship between tissue and plasma PD-L1 expression and to determine its diagnostic and prognostic value.METHODS:PD-L1 expression in tissue was assessed by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA), and sPD-L1 concentration in plasma was quantified by ELISA. The levels of the CD274 gene, which encodes for PD-L1 protein, were examined as part of bulk tissue RNA-sequencing analyses. Additionally, we evaluated the association between sPD-L1 levels and various laboratory parameters, disease characteristics, and patient outcomes.RESULTS:GC patients had significantly higher levels of sPD-L1 in their plasma (71.69 pg/mL) compared to healthy controls (35.34 pg/mL) (p < 0.0001). Moreover, sPD-L1 levels were significantly correlated with tissue PD-L1 protein, CD274 mRNA expression, larger tumor size, advanced tumor stage, and lymph node metastasis. Elevated sPD-L1 levels (> 103.5 ng/mL) were associated with poor overall survival (HR = 2.16, 95%CI 1.15-4.08, p = 0.017). Furthermore, intratumoral neutrophil and dendritic cell levels were directly correlated with plasma sPD-L1 concentration in the GC patients.CONCLUSIONS:sPD-L1 was readily measurable in GC patients, and its level was associated with GC tissue PD-L1 expression, greater inflammatory cell infiltration, disease progression, and survival. Thus, sPD-L1 may be a useful minimally invasive diagnostic and prognostic biomarker in GC patients.
DOI	10.1007/s10120-023-01429-7
PMID	37668884