ミヤタ　マリコ   Miyata Mariko   宮田　麻理子    所属   医学部 医学科    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Developmental refinement of the active zone nanotopography and axon wiring at the somatosensory thalamus
掲載誌名	正式名：Cell Reports ISSNコード：22111247
掲載区分	国内
出版社	Cell Press
巻・号・頁	43(10),pp.114770-114770
著者・共著者	https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01121-5
担当区分	最終著者,責任著者
発行年月	2024/10/22
概要	Functional refinement of neural circuits is a crucial developmental process in the brain. However, how synaptic maturation and axon wiring proceed cooperatively to establish reliable signal transmission is unclear. Here, we combined nanotopography of release machinery at the active zone (AZ), nanobiophysics of neurotransmitter release, and single-neuron reconstruction of axon arbors of lemniscal fibers (LFs) in the developing mouse somatosensory thalamus. With development, the cluster of Cav2.1 enlarges and translocates closer to vesicle release sites inside the bouton, and LFs drastically shrink their arbors and form larger boutons on the perisomatic region of target neurons. Experimentally constrained simulations show that the nanotopography of mature synapses enables not only rapid vesicular release but also reliable transmission following repetitive firing. Sensory deprivation impairs the developmental shift of molecular nanotopography and axon wiring. Thus, we uncovered the cooperative nanotopographical and morphological mechanisms underlying the developmental establishment of reliable synaptic transmission.
DOI	https://doi.org/10.1016/j.celrep.2024.114770