マルヤマ　タクマ   Maruyama Takuma   丸山　拓真    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	ON and OFF starburst amacrine cells are controlled by distinct cholinergic pathways.
掲載誌名	正式名：The Journal of general physiology 略　 称：J Gen Physiol ISSNコード：15407748/00221295
掲載区分	国外
巻・号・頁	156(8),pp.e202413550
著者・共著者	Gangi Mie, Maruyama Takuma, Ishii Toshiyuki, Kaneda Makoto
担当区分	2nd著者
発行年月	2024/08
概要	Cholinergic signaling in the retina is mediated by acetylcholine (ACh) released from starburst amacrine cells (SACs), which are key neurons for motion detection. SACs comprise ON and OFF subtypes, which morphologically show mirror symmetry to each other. Although many physiological studies on SACs have targeted ON cells only, the synaptic computation of ON and OFF SACs is assumed to be similar. Recent studies demonstrated that gene expression patterns and receptor types differed between ON and OFF SACs, suggesting differences in their functions. Here, we compared cholinergic signaling pathways between ON and OFF SACs in the mouse retina using the patch clamp technique. The application of ACh increased GABAergic feedback, observed as postsynaptic currents to SACs, in both ON and OFF SACs; however, the mode of GABAergic feedback differed. Nicotinic receptors mediated GABAergic feedback in both ON and OFF SACs, while muscarinic receptors mediated GABAergic feedback in ON SACs only in adults. Neither tetrodotoxin, which blocked action potentials, nor LY354740, which blocked neurotransmitter release from SACs, eliminated ACh-induced GABAergic feedback in SACs. These results suggest that ACh-induced GABAergic feedback in ON and OFF SACs is regulated by different feedback mechanisms in adults and mediated by non-spiking amacrine cells other than SACs.
DOI	10.1085/jgp.202413550
PMID	38836782