タカギ　リヨウ   Takagi Riyou   高木　亮    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Dual-color FISH analyses of xenogeneic human fibroblast sheets transplanted to repair lung pleural defects in an immunocompromised rat model.
掲載誌名	正式名：BMC research notes 略　 称：BMC Res Notes ISSNコード：17560500/17560500
掲載区分	国外
巻・号・頁	17(1),pp.139
著者・共著者	KANZAKI Masato†*, TAKAGI Ryo, MITSUBOSHI shota, SHIDEI Hiroaki, ISAKA Tamami, YAMATO Masayuki
担当区分	2nd著者
発行年月	2024/05/15
概要	BACKGROUND:Pulmonary air leaks (PALs) due to visceral pleura injury during surgery is frequently observed after pulmonary resections and the complication is difficult to avoid in thoracic surgery. The development of postoperative PALs is the most common cause of prolonged hospitalization. Previously, we reported that PALs sealants using autologous dermal fibroblast sheets (DFSs) harvested from temperature-responsive culture dishes successfully closed intraoperative PALs during lung resection.OBJECTIVE:In this study, we investigated the fate of human DFSs xenogenetically transplanted onto lung surfaces to seal PALs of immunocompromised rat. Dual-color FISH analyses of human fibroblast was employed to detect transplantation human cells on the lung surface.RESULTS:One month after transplantation, FISH analyses revealed that transplanted human fibroblasts still composed a sheet-structure, and histology also showed that beneath the sheet's angiogenesis migrating into the sheets was observed from the recipient tissues. FISH analyses revealed that even at 3 months after transplantation, the transplanted human fibroblasts still remained in the sheet. Dual-color FISH analyses of the transplanted human fibroblasts were sparsely present as a result of the cells reaching the end of their lifespan, the cells producing extracellular matrix, and remained inside the cell sheet and did not invade the lungs of the host.CONCLUSIONS:DFS-transplanted human fibroblasts showed that they are retained within cell sheets and do not invade the lungs of the host.
DOI	10.1186/s13104-024-06792-x
PMID	38750547