イシヅカ　ツトム   Ishidzuka Tsutomu   石塚　敏    所属   その他 その他    職種   臨床検査副技師長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	5-Aminolevulinic acid-based photodynamic diagnosis for detection of urothelial carcinoma cells in bladder washing sediment suspension: A pilot study.
掲載誌名	正式名：Photodiagnosis and photodynamic therapy 略　 称：Photodiagnosis Photodyn Ther ISSNコード：18731597/15721000
掲載区分	国外
巻・号・頁	40,pp.103072
著者・共著者	Yokoyama Takashi, Toguchi Makoto, Iizuka Junpei, Horita Shigeru, Ishizuka Tsutomu, Chu-Su Yu, Nagashima Yoji, Takagi Toshio, Tanabe Kazunari, Tokuoka Yoshikazu
発行年月	2022/12
概要	BACKGROUND:Bladder cancer is a common malignant disease in developed countries. Early detection of malignancy is important using urine cytology. The 5-aminolevulinic acid (ALA)-based photodynamic diagnosis (ALA-PDD) has not been routinely applied in urine cytology analysis yet, although it has been well accepted for tumor lesion marking in cystoscopy.METHODS:A total of eight volunteers were enrolled in this study. The cells of sediment suspension from bladder washing fluid and random urine were stained by ALA-induced protoporphyrin IX (ALA-PpIX) and the fluorescent intensity of ALA-PpIX was analyzed by ImageJ.RESULTS:The cutoff value of fluorescent intensity was 90.260 per pixel. The proposed protocol provided an objective fluorescent intensity for evaluation. Sensitivity was 0.931 and specificity was 1.000.CONCLUSIONS:The staining procedure applied was ALA-PpIX for suspicious cells in the cellular suspension from bladder wash fluid and random urine. ImageJ was applied to the objective measurement for the fluorescent intensity of the stained cells. The cutoff value for the positive result was 90.260 per pixel. Therefore, the protocol proposed in this study provides a potential means to enhance accuracy for urine cytology analysis.
DOI	10.1016/j.pdpdt.2022.103072
PMID	35998879