タカギ　リヨウ   Takagi Riyou   高木　亮    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3.
掲載誌名	正式名：Stem cell research & therapy 略　 称：Stem Cell Res Ther ISSNコード：17576512/17576512
掲載区分	国外
巻・号・頁	14(1),pp.242
著者・共著者	KOBORI Chie†, TAKAGI Ryo, YOKOMIZA Ryo, YOSHIHARA Sakie, Mori Mai, TAKAHASHI Hiroto, Javaregowda Palaksha Kanive, AKIYAMA Tomohiko, KO Minoru S H, KISHI Kazuo, UMEZAWA Akihiro*
発行年月	2023/09
概要	BACKGROUND:Melanocytes are an essential part of the epidermis, and their regeneration has received much attention because propagation of human adult melanocytes in vitro is too slow for clinical use. Differentiation from human pluripotent stem cells to melanocytes has been reported, but the protocols to produce them require multiple and complex differentiation steps.METHOD:We differentiated human embryonic stem cells (hESCs) that transiently express JMJD3 to pigmented cells. We investigated whether the pigmented cells have melanocytic characteristics and functions by qRT-PCR, immunocytochemical analysis and flow cytometry. We also investigated their biocompatibility by injecting the cells into immunodeficient mice for clinical use.RESULT:We successfully differentiated and established a pure culture of melanocytes. The melanocytes maintained their growth rate for a long time, approximately 200 days, and were functional. They exhibited melanogenesis and transfer of melanin to peripheral keratinocytes. Moreover, melanocytes simulated the developmental processes from melanoblasts to melanocytes. The melanocytes had high engraftability and biocompatibility in the immunodeficient mice.CONCLUSION:The robust generation of functional and long-lived melanocytes are key to developing clinical applications for the treatment of pigmentary skin disorders.
DOI	10.1186/s13287-023-03479-1
PMID	37679843