セキネ　ヒデカズ   Sekine Hidekazu   関根　秀一    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research.
掲載誌名	正式名：Cells 略　 称：Cells ISSNコード：20734409/20734409
掲載区分	国外
巻・号・頁	13(3),pp.228
著者・共著者	MATSUI Kenji†, SEKINE Hidekazu, ISHIKAWA Jun, ENOSAWA Shin, MATSUMOTO Naoto, INAGE Yuka, KINOSHITA Yoshitaka, MORIMOTO Keita, YAMAMOTO Shutaro, KODA Nagisa, YAMANAKA Shuichiro, YOKOO Takashi, KOBAYASHI Eiji*
担当区分	2nd著者
発行年月	2024/01/25
概要	Human pluripotent stem cells have been employed in generating organoids, yet their immaturity compared to fetal organs and the limited induction of all constituent cell types remain challenges. Porcine fetal progenitor cells have emerged as promising candidates for co-culturing with human progenitor cells in regeneration and xenotransplantation research. This study focused on identifying proper preservation methods for porcine fetal kidneys, hearts, and livers, aiming to optimize their potential as cell sources. Extracted from fetal microminiature pigs, these organs were dissociated before and after cryopreservation-thawing, with subsequent cell quality evaluations. Kidney cells, dissociated and aggregated after vitrification in a whole-organ form, were successfully differentiated into glomeruli and tubules in vivo. In contrast, freezing hearts and livers before dissociation yielded suboptimal results. Heart cells, frozen after dissociation, exhibited pulsating heart muscle cells similar to non-frozen hearts. As for liver cells, we developed a direct tissue perfusion technique and successfully obtained highly viable liver parenchymal cells. Freezing dissociated liver cells, although inferior to their non-frozen counterparts, maintained the ability for colony formation. The findings of this study provide valuable insights into suitable preservation methods for porcine fetal cells from kidneys, hearts, and livers, contributing to the advancement of regeneration and xenotransplantation research.
DOI	10.3390/cells13030228
PMID	38334620