コダマ　タカシ   Kodama Takashi   児玉　貴史    所属   医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Neuronal classification and marker gene identification via single-cell expression profiling of brainstem vestibular neurons subserving cerebellar learning.
掲載誌名	正式名：The Journal of neuroscience : the official journal of the Society for Neuroscience 略　 称：J Neurosci ISSNコード：15292401/02706474
掲載区分	国外
巻・号・頁	32(23),pp.7819-31
著者・共著者	Kodama Takashi, Guerrero Shiloh, Shin Minyoung, Moghadam Seti, Faulstich Michael, du Lac Sascha
担当区分	筆頭著者
発行年月	2012/06
概要	Identification of marker genes expressed in specific cell types is essential for the genetic dissection of neural circuits. Here we report a new strategy for classifying heterogeneous populations of neurons into functionally distinct types and for identifying associated marker genes. Quantitative single-cell expression profiling of genes related to neurotransmitters and ion channels enables functional classification of neurons; transcript profiles for marker gene candidates identify molecular handles for manipulating each cell type. We apply this strategy to the mouse medial vestibular nucleus (MVN), which comprises several types of neurons subserving cerebellar-dependent learning in the vestibulo-ocular reflex. Ion channel gene expression differed both qualitatively and quantitatively across cell types and could distinguish subtle differences in intrinsic electrophysiology. Single-cell transcript profiling of MVN neurons established six functionally distinct cell types and associated marker genes. This strategy is applicable throughout the nervous system and could facilitate the use of molecular genetic tools to examine the behavioral roles of distinct neuronal populations.
DOI	10.1523/JNEUROSCI.0543-12.2012
PMID	22674258