モリモト　サトシ   Morimoto Satoshi   森本　聡    所属   医学部 医学科（東京女子医科大学病院）    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Thyroid hormone activation in vascular smooth muscle cells is negatively regulated by glucocorticoid.
掲載誌名	正式名：Thyroid : official journal of the American Thyroid Association 略　 称：Thyroid ISSNコード：15579077/10507256
掲載区分	国外
巻・号・頁	19(7),pp.755-63
著者・共著者	Toyoda Nagaoki, Yasuzawa-Amano Saori, Nomura Emiko, Yamauchi Azusa, Nishimura Kumiko, Ukita Chizuko, Morimoto Satoshi, Kosaki Atsushi, Iwasaka Toshiji, Harney John W, Larsen P Reed, Nishikawa Mitsushige
発行年月	2009/07
概要	BACKGROUND:Type 2 iodothyronine deiodinase (D2) catalyzes the production of triiodothyronine from thyroxine. D2 is present in rat aorta media, and there is a circadian variation in the D2 expression. In rat aorta media, the D2 activity exhibited the maximal value at 1200 hour and low value between 1800 and 2400 hour. To understand the mechanisms that induce the circadian variation in the D2 expression, we examined the effects of glucocorticoid on the D2 activity and mRNA in rat aorta media and cultured vascular smooth muscle cells (VSMCs).METHODS:The effects of intrinsic and extrinsic glucocorticoid on the D2 activity and mRNA in rat aorta media were studied using metyrapone, a corticosterone synthesis inhibitor, and dexamethasone (DEX). Further, the effects of DEX on D2 expression were studied using the cultured rat VSMCs.RESULTS:The trough values of D2 activity and mRNA at 2100 hour were increased by the treatment with metyrapone. On the other hand, the peak values of D2 activity and mRNA were decreased by the treatment with DEX. D2 activity and mRNA in cultured rat VSMCs were increased by the addition of 10(-3) M dibutyryl cyclic adenosine monophosphate [(Bu)(2)cAMP]. The increments were reduced by coincubation with 10(-6) M DEX.CONCLUSIONS:These results suggest that glucocorticoids might directly suppress the D2 expression in rat VSMCs induced by a cAMP-dependent mechanism.
DOI	10.1089/thy.2009.0044
PMID	19508118