キハラ　ユウキ   Kihara Yuuki   木原　祐希    所属   医学部 医学科（東京女子医科大学病院）    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death.
掲載誌名	正式名：Regenerative therapy 略　 称：Regen Ther ISSNコード：23523204/23523204
掲載区分	国外
巻・号・頁	21,pp.486-493
著者・共著者	Kihara Yuki†, Tanaka Yukie, Ikeda Masanari, Homma Jun, Takagi Ryo, Ishigaki Keiko, Yamanouchi Keitaro, Honda Hiroaki, Nagata Satoru, Yamato Masayuki*
担当区分	筆頭著者
発行年月	2022/12
概要	INTRODUCTION:Duchenne muscular dystrophy (DMD) is a progressive disease that leads to damage of muscle and myocardium due to genetic abnormalities in the dystrophin gene. In utero cell transplantation that might facilitate allogenic transplantation is worth considering to treat this disease.METHODS:We performed allogeneic in utero transplantation of GFP-positive myoblasts and adipose-derived mesenchymal stem cells into murine DMD model animals. The transplantation route in this study was fetal intraperitoneal transplantation and transplacental transplantation. Transplanted animals were examined at 4-weeks old by immunofluorescence staining and RT-qPCR.RESULTS:No GFP-positive cells were found by immunofluorescence staining of skeletal muscle and no GFP mRNA was detected by RT-qPCR in any animal, transplantation method and cell type. Compared with previous reports, myoblast transplantation exhibited an equivalent mortality rate, but adipose-derived stem cell (ASC) transplantation produced a higher mortality rate.CONCLUSIONS:In utero transplantation of myoblasts or ASCs to murine models of DMD does not lead to engraftment and, in ASC transplantation primarily, frequently results in fetal death.
DOI	10.1016/j.reth.2022.10.003
PMID	36313392