セキネ　ヒデカズ   Sekine Hidekazu   関根　秀一    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet.
掲載誌名	正式名：Regenerative therapy 略　 称：Regen Ther ISSNコード：23523204/23523204
掲載区分	国外
巻・号・頁	24,pp.561-567
著者・共著者	Yamamoto Shutaro†, Matsui Kenji, Kinoshita Yoshitaka, Hiroshi Sasaki, Sekine Hidekazu, Saito Yatsumu, Nakayama Yasuhide, Kume Haruki, Kimura Takahiro, Yokoo Takashi, Kobayashi Eiji*
発行年月	2023/12
概要	INTRODUCTION:Ureteral injuries require surgical intervention as they lead to loss of renal function. The current reconstructive techniques for long ureteral defects are problematic. Consequently, this study aimed to reconstruct the ureter in a rat model using subcutaneously prepared autologous collagen tubes (Biotubes).METHODS:The lower ureter of LEW/SsNSlc rats was ligated to dilate the ureter to make anastomosis easier, and reconstruction was performed six days later by anastomosing the dilated ureter and bladder with a Biotube that was prepared subcutaneously in syngeneic rats. Some rats underwent left nephrectomy and ureter reconstruction simultaneously as negative controls to evaluate the effects of urine flow on patency. The other rats were divided into three groups as follows: a group in which the ureter was reconstructed with the Biotube alone, a group in which cardiomyocyte sheets made from the neonatal hearts of syngeneic rats were wrapped around the Biotube, and a group in which an adipose-derived stem cell sheets made from the inguinal fat of adult syngeneic rats were wrapped. Contrast-enhanced computed tomography and pathological evaluations were performed two weeks after reconstruction.RESULT:In the Biotube alone group, all tubes were occluded and hydronephrosis developed, whereas the urothelium regenerated beyond the anastomosis when the left kidney was not removed, suggesting that urothelial epithelial spread occurred with urinary flow. The patency of the ureteral lumen was obtained in some rats in the cardiomyocyte sheet covered group, whereas stricture or obstruction of the reconstructed ureter was observed in all rats in the other groups. Pathological evaluation revealed a layered urothelial structure in the cardiomyocyte sheet covered group, although only a small amount of cardiomyocyte sheets remained.CONCLUSION:Urinary flow may support the epithelial spread of the urothelium into the reconstructed ureter. Neonatal rat cardiomyocyte sheets suppo
DOI	10.1016/j.reth.2023.10.001
PMID	37868722