シミズ　タツヤ   Shimizu Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids.
掲載誌名	正式名：Archives of microbiology 略　 称：Arch Microbiol ISSNコード：1432072X/03028933
掲載区分	国外
出版社	Springer nature
巻・号・頁	205(7),pp.266
総ページ数	17
著者・共著者	HARAGUCHI Yuji†*, KATO Yuichi, INABE Kosuke, KONDO Akihiko, HASANUMA Tomohisa, SHIMIZU Tatsuya*
担当区分	最終著者,責任著者
発行年月	2023/06/16
概要	Recently, we reported a circular cell culture (CCC) system using microalgae and animal muscle cells for sustainable culture food production. However, lactate accumulation excreted by animal cells in the system characterized by medium reuse was a huge problem. To solve the problem, as an advanced CCC, we used a lactate-assimilating cyanobacterium Synechococcus sp. PCC 7002, using gene-recombination technology that synthesises pyruvate from lactate. We found that the cyanobacteria and animal cells mutually exchanged substances via their waste media: (i) cyanobacteria used lactate and ammonia excreted by animal muscle cells, and (ii) the animal cells used pyruvate and some amino acids excreted by the cyanobacteria. Because of this, animal muscle C2C12 cells were amplified efficiently without animal serum in cyanobacterial culture waste medium in two cycles (first cycle: 3.6-fold; second cycle: 3.9-fold/three days-cultivation) using the same reuse medium. We believe that this advanced CCC system will solve the problem of lactate accumulation in cell culture and lead to efficient cultured food production.
DOI	10.1007/s00203-023-03607-8
PMID	37328623