イシイ　ヤスオ   Ishii Yasuo   石井　泰雄    所属   医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Live imaging of avian epiblast and anterior mesendoderm grafting reveals the complexity of cell dynamics during early brain development.
掲載誌名	正式名：Development (Cambridge, England) 略　 称：Development ISSNコード：14779129/09501991
掲載区分	国外
巻・号・頁	149(6),pp.dev1999999
著者・共著者	Yoshihi Koya†, Kato Kagayaki, Iida Hideaki, Teramoto Machiko, Kawamura Akihito, Watanabe Yusaku, Nunome Mitsuo, Nakano Mikiharu, Matsuda Yoichi, Sato Yuki, Mizuno Hidenobu, Iwasato Takuji, Ishii Yasuo, Kondoh Hisato*
発行年月	2022/03
概要	Despite previous intensive investigations on epiblast cell migration in avian embryos during primitive streak development before stage (st.) 4, this migration at later stages of brain development has remained uninvestigated. By live imaging of epiblast cells sparsely labeled with green fluorescence protein, we investigated anterior epiblast cell migration to form individual brain portions. Anterior epiblast cells from a broad area migrated collectively towards the head axis during st. 5-7 at a rate of 70-110 µm/h, changing directions from diagonal to parallel and forming the brain portions and abutting head ectoderm. This analysis revised the previously published head portion precursor map in anterior epiblasts at st. 4/5. Grafting outside the brain precursor region of mCherry-expressing nodes producing anterior mesendoderm (AME) or isolated AME tissues elicited new cell migration towards ectopic AME tissues. These locally convergent cells developed into secondary brains with portions that depended on the ectopic AME position in the anterior epiblast. Thus, anterior epiblast cells are bipotent for brain/head ectoderm development with given brain portion specificities. A brain portion potential map is proposed, also accounting for previous observations.
DOI	10.1242/dev.199999
PMID	35132990