モリ　トシユキ   Mori Toshiyuki   森　稔幸    所属   医学部 医学科    職種   特任助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Sequence-dependent heterochromatin formation in the human malaria parasite Plasmodium falciparum.
掲載誌名	正式名：Heliyon 略　 称：Heliyon ISSNコード：24058440/24058440
掲載区分	国外
巻・号・頁	9(9),pp.e19164
著者・共著者	Mori Toshiyuki, Nakashima Mai
発行年月	2023/08
概要	The human malaria parasite Plasmodium falciparum represses transcription of the gene encoding AP2-G, which is the master regulator of germ cell differentiation, via heterochromatin condensation following histone H3 lysine 9 trimethylation (H3K9me3). Although H3K9me3-marked heterochromatin is typically constitutive and its establishment depends on the RNA interference (RNAi) pathway in fission yeast centromeres, malaria parasites lack molecular members essential for RNAi. We developed a strategy to assess heterochromatin establishment on artificial chromosomes introduced into P. falciparum. We show that a particular DNA sequence in the AP2-G promoter is able to induce de novo H3K9me3 nucleosome deposition. In addition, we also found that the AP2-G promoter contains a distinct element required in maintenance of the repression memory. Thus, we speculate that malaria parasites have evolutionarily acquired a sequence-dependent establishment system of non-constitutive, i.e. facultative, H3K9me3-marked heterochromatin.
DOI	10.1016/j.heliyon.2023.e19164
PMID	37681121