モリ トシユキ
森 稔幸 所属 医学部 医学科 職種 特任助教 |
|
論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Sequence-dependent heterochromatin formation in the human malaria parasite Plasmodium falciparum. |
掲載誌名 | 正式名:Heliyon 略 称:Heliyon ISSNコード:24058440/24058440 |
掲載区分 | 国外 |
巻・号・頁 | 9(9),pp.e19164 |
著者・共著者 | Mori Toshiyuki, Nakashima Mai |
発行年月 | 2023/08 |
概要 | The human malaria parasite Plasmodium falciparum represses transcription of the gene encoding AP2-G, which is the master regulator of germ cell differentiation, via heterochromatin condensation following histone H3 lysine 9 trimethylation (H3K9me3). Although H3K9me3-marked heterochromatin is typically constitutive and its establishment depends on the RNA interference (RNAi) pathway in fission yeast centromeres, malaria parasites lack molecular members essential for RNAi. We developed a strategy to assess heterochromatin establishment on artificial chromosomes introduced into P. falciparum. We show that a particular DNA sequence in the AP2-G promoter is able to induce de novo H3K9me3 nucleosome deposition. In addition, we also found that the AP2-G promoter contains a distinct element required in maintenance of the repression memory. Thus, we speculate that malaria parasites have evolutionarily acquired a sequence-dependent establishment system of non-constitutive, i.e. facultative, H3K9me3-marked heterochromatin. |
DOI | 10.1016/j.heliyon.2023.e19164 |
PMID | 37681121 |