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シミズ タツヤ
Shimizu Tatsuya
清水 達也 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 教授 |
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| 論文種別 | 原著 |
| 言語種別 | 日本語 |
| 査読の有無 | 査読あり |
| 表題 | Tricultured Cell Sheets Develop into Functional Pancreatic Islet Tissue with a Vascular Network. |
| 掲載誌名 | 正式名：Tissue engineering. Part A 略 称：Tissue Eng Part A ISSNコード：1937335X/19373341 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 29(7-8),211-224頁 |
| 著者・共著者 | HOMMAJun†, SEKINEHidekazu*, SHIMIZUTatsuya |
| 担当区分 | 最終著者 |
| 発行年月 | 2023/04 |
| 概要 | Methods to induce islet β-cells from induced pluripotent stem cells or embryonic stem cells have been established. However, islet β-cells are susceptible to apoptosis under hypoxic conditions, so the technique used to transplant β-cells must maintain the viability of cells in vivo. This study describes the development of a tricultured cell sheet, which was made by coculturing islet β-cells, vascular endothelial cells, and mesenchymal stem cells for 1 day. The islet β-cells in the tricultured cell sheet self-organized into islet-like structures surrounded by a dense vascular network in vitro. Triple-layered tricultured cell sheets engrafted well after transplantation in vivo and developed into insulin-secreting tissue with abundant blood vessels and a high density of islet β-cells. We anticipate that the tricultured cell sheet could be used as an in vitro pseudo-islet model for pharmaceutical testing and may have potential for development into transplantable grafts for use in regenerative medicine. Impact statement This research assessed whether tricultured cell sheets containing islet β-cells, vascular endothelial cells, and mesenchymal stem cells were able to form islet tissue. There were two main findings. First, the islet β-cells in the tricultured cell sheet self-organized into islet structures surrounded by a dense vascular network in vitro. Second, triple-layered tricultured sheets engrafted well onto rat muscle and developed into insulin-secreting tissue with an abundance of blood vessels. The tricultured cell sheet could be used as a pseudo-islet model for pharmaceutical testing and may have potential for development into a transplantable graft for application in the clinical setting. |
| DOI | 10.1089/ten.TEA.2022.0167 |
| PMID | 36565034 |