ホンマ　ジユン   Honma Jiyun   本間　順    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	日本語
査読の有無	査読あり
表題	Tricultured Cell Sheets Develop into Functional Pancreatic Islet Tissue with a Vascular Network.
掲載誌名	正式名：Tissue engineering. Part A 略　 称：Tissue Eng Part A ISSNコード：1937335X/19373341
掲載区分	国外
巻・号・頁	29(7-8),211-224頁
著者・共著者	HOMMAJun†, SEKINEHidekazu*, SHIMIZUTatsuya
担当区分	筆頭著者
発行年月	2023/04
概要	Methods to induce islet β-cells from induced pluripotent stem cells or embryonic stem cells have been established. However, islet β-cells are susceptible to apoptosis under hypoxic conditions, so the technique used to transplant β-cells must maintain the viability of cells in vivo. This study describes the development of a tricultured cell sheet, which was made by coculturing islet β-cells, vascular endothelial cells, and mesenchymal stem cells for 1 day. The islet β-cells in the tricultured cell sheet self-organized into islet-like structures surrounded by a dense vascular network in vitro. Triple-layered tricultured cell sheets engrafted well after transplantation in vivo and developed into insulin-secreting tissue with abundant blood vessels and a high density of islet β-cells. We anticipate that the tricultured cell sheet could be used as an in vitro pseudo-islet model for pharmaceutical testing and may have potential for development into transplantable grafts for use in regenerative medicine. Impact statement This research assessed whether tricultured cell sheets containing islet β-cells, vascular endothelial cells, and mesenchymal stem cells were able to form islet tissue. There were two main findings. First, the islet β-cells in the tricultured cell sheet self-organized into islet structures surrounded by a dense vascular network in vitro. Second, triple-layered tricultured sheets engrafted well onto rat muscle and developed into insulin-secreting tissue with an abundance of blood vessels. The tricultured cell sheet could be used as a pseudo-islet model for pharmaceutical testing and may have potential for development into a transplantable graft for application in the clinical setting.
DOI	10.1089/ten.TEA.2022.0167
PMID	36565034