ハラ　ツカサ   Hara Tsukasa   原　司    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   特任助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Mitochondrial respiratory chain supercomplexes in human mononuclear leukocytes
掲載誌名	正式名：International Journal of Analytical Bio-Science 略　 称：Int. J. Anal. BioSci. ISSNコード：21877912/21877920
掲載区分	国外
出版社	(NPO)生物試料分析科学会
巻・号・頁	4(1),pp.6-12
総ページ数	7
著者・共著者	Hara Tsukasa, Amagai Ryosuke, Koga Michinobu, Okado-Matsumoto Ayako
担当区分	筆頭著者
発行年月	2016/03
概要	An association of mitochondrial respiratory chain complexes is called a supercomplex. These supercomplexes enhance energy production and reduce electron leaking. Destabilization of supercomplexes may increase oxidative stress and mitochondrial dysfunction, and aging is a trigger for mitochondrial dysfunction. We used high-resolution clear native-polyacrylamide gel electrophoresis (hrCN-PAGE) and two-dimensional hrCN/SDS-PAGE to examine supercomplex formation in human mononuclear leukocytes and estimate complex I proportions for supercomplexes. The results were then compared in relation to sex and age group. Supercomplex clusters were more stable in females than in males. Larger supercomplexes were more stable among subjects aged 55–67 years than in those aged 21–25 years; smaller supercomplexes were less stable among subjects aged 55–67 years than in those aged 21–25 years.
文献番号	2016296500