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ヤマグチ シゲキ
Yamaguchi Shigeki
山口 茂樹 所属 医学部 医学科（東京女子医科大学病院） 職種 教授・基幹分野長 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読なし |
| 表題 | Transplantation of hybrid adipose-derived stem cell sheet with autologous peritoneum: An in vivo feasibility study. |
| 掲載誌名 | 正式名：Heliyon 略 称：Heliyon ISSNコード：24058440/24058440 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 9(2),pp.e12992 |
| 著者・共著者 | MATSUO Natsuki, OHKI Takeshi, AOYAMA Shota, YAMAGUCHI SHIGEKI, ITABASHI Michio, EGAWA Hiroto, YAMAMOTO Masakazu |
| 発行年月 | 2023/02 |
| 概要 | INTRODUCTION:In regenerative medicine, cell sheet engineering has various advantages, including the retention of cells at the transplantation site for a longer period and the local delivery of growth factors and cytokines. Adipose-derived stem cell (ASC) is widely used owing to their various functions such as wound healing, immunomodulation, and nerve regeneration, in addition to their ability to differentiate into adipocytes, chondrocytes, and osteoblasts. ASC sheet generated using cell sheet engineering is considered effective in preventing anastomotic leakage, a serious postoperative complication in gastrointestinal surgery. However, the ASC sheet is too soft, thin, and brittle to handle with laparoscopic forceps during the operation. Therefore, we considered using the peritoneum, which is stiff and easy to collect while operating, as an alternative support. In this study, we explored the feasibility of using the peritoneum as a support for the precise transplantation of ASC sheets during surgery.METHODS:ASCs were isolated from the subcutaneous fat of the inguinal region of Sprague-Dawley (SD) transgenic rats expressing green fluorescent protein. ASCs were cultured until passage 3, seeded in temperature-responsive culture dishes, and the resulting ASC sheet was harvested at more than 80% confluency. Non-transgenic SD rats were used for transplant experiments. The wall peritoneum was harvested from SD rats following laparotomy, and hybrid adipose-derived stem cell (HASC) sheet was prepared by laminating the peritoneum with ASC sheet. The cell sheets were transplanted on the backs of SD rats following the incision. On post-transplantation days 3 and 7, the specimens were extracted. ASC and HASC sheets were then compared macroscopically and histopathologically.RESULTS:HASC sheet transplantation was macroscopically and histopathologically more effective than ASC sheet transplantation. The peritoneum provided sufficient stiffness as a support for precise transplantati |
| DOI | 10.1016/j.heliyon.2023.e12992 |
| PMID | 36747528 |