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イワシタ ハナコ
Iwashita Hanako
岩下 華子 所属 医学部 医学科 職種 助教 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Molecular epidemiology of Cryptosporidium spp. in an agricultural area of northern Vietnam: A community survey. |
| 掲載誌名 | 正式名：Parasitology international 略 称：Parasitol Int ISSNコード：18730329/13835769 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 83,pp.102341 |
| 著者・共著者 | Iwashita Hanako, Takemura Taichiro, Tokizawa Asako, Sugamoto Tetsuhiro, Thiem Vu Dinh, Nguyen Tuan Hai, Pham Tho Duc, Pham Anh Hong Quynh, Doan Hang Thi, Tran Na Ly, Yamashiro Tetsu |
| 発行年月 | 2021/08 |
| 概要 | The purpose of this study was to investigate the occurrence of Cryptosporidium infection and the potential for transmission of Cryptosporidium spp. between animals and humans in northern Vietnam. A total of 2715 samples (2120 human diarrheal samples, 471 human non-diarrheal samples, and 124 animal stool samples) were collected through our community survey in an agricultural area. All samples were tested for Cryptosporidium spp. by direct immunofluorescence assay (DFA) using a fluorescent microscope. DNA extraction, PCR amplification of three genes (COWP, SSU-rRNA, and GP60), and sequencing analysis were performed to identify Cryptosporidium spp. Of 2715 samples, 15 samples (10 diarrheal samples, 2 non-diarrheal samples, and 3 animal stool samples) tested positive by PCR for the COWP gene. Three species of Cryptosporidium spp. were identified as C. canis (from six human diarrheal samples, two human non-diarrheal samples, and one dog sample), C. hominis (from four human diarrheal samples), and C. suis (from two pig samples) by sequencing the amplified COWP and/or SSU-rRNA genes. In terms of C. hominis, the GP60 subtype IeA12G3T3 was detected in all four human diarrheal samples. Although the number of positive samples was very small, our epidemiological data showed that the emerging pattern of each of the three species (C. canis, C. hominis, and C. suis) was different at this study site. While C. hominis and C. suis were only detected in human and pig samples, respectively, C. canis was detected in samples from both dogs and humans. We suspect that C. canis infections in humans at this study site may be due to environmental contamination with animal and human feces. |
| DOI | 10.1016/j.parint.2021.102341 |
| PMID | 33819572 |