オオサワ シゲヒト   Oosawa Shigehito
  大澤 重仁
   所属   医学研究科 医学研究科 (医学部医学科をご参照ください)
   職種   非常勤講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Polyplex micelles with double-protective compartments of hydrophilic shell and thermoswitchable palisade of poly (oxazoline)-based block copolymers for promoted gene transfection
掲載誌名 正式名:Biomacromolecules
略  称:Biomacromolecules
ISSNコード:15257797/15264602
掲載区分国外
巻・号・頁 17(1),pp.354-361
総ページ数 8
著者・共著者 Shigehito Osawa, Kensuke Osada, Shigehiro Hiki, Anjaneyulu Dirisala, Takehiko Ishii, Kazunori Kataoka
担当区分 筆頭著者
発行年月 2016/01/11
概要 Improving the stability of polyplex micelles under physiological conditions is a critical issue for promoting gene transfection efficiencies. To this end, hydrophobic palisade was installed between the inner core of packaged plasmid DNA (pDNA) and the hydrophilic shell of polyplex micelles using a triblock copolymer consisting of hydrophilic poly(2-ethyl-2-oxazoline), thermoswitchable amphiphilic poly(2-n-propyl-2-oxazoline) (PnPrOx) and cationic poly(l-lysine). The two-step preparation procedure, mixing the triblock copolymer with pDNA below the lower critical solution temperature (LCST) of PnPrOx, followed by incubation above the LCST to form a hydrophobic palisade of the collapsed PnPrOx segment, induced the formation of spatially aligned hydrophilic–hydrophobic double-protected polyplex micelles. The prepared polyplex micelles exhibited significant tolerance against attacks from nuclease and polyanions compared to those without hydrophobic palisades, thereby promoting gene transfection. These results corroborated the utility of amphiphilic poly(oxazoline) as a molecular thermal switch to improve the stability of polyplex gene carriers relevant for physiological applications.
DOI 10.1021/acs.biomac.5b01456