オオサワ　シゲヒト   Oosawa Shigehito   大澤　重仁    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Interpenetrating polymer network hydrogels via a one-pot and in situ gelation system based on peptide self-assembly and orthogonal cross-linking for tissue regeneration
掲載誌名	正式名：Chemistry of materials : a publication of the AmericanChemical Society. 略　 称：Chem Mater ISSNコード：08974756/15205002
掲載区分	国外
巻・号・頁	32(6),pp.2353-2364
総ページ数	12
著者・共著者	Shohei Ishikawa, Kazutoshi Iijima, Daisuke Matsukuma, Yukiyo Asawa, Kazuto Hoshi, Shigehito Osawa, Hidenori Otsuka
担当区分	責任著者
発行年月	2020/02/19
概要	Construction of the interpenetrating polymer network (IPN) in hydrogels has received increased attention because it not only improves their mechanical properties but also mimics the extracellular matrix, which can be used as cell culturing scaffolds for tissue engineering. Usually, IPN gels are prepared using separated procedures, in which primal networks, followed by other networks, are formed by adding chemical reagents or subjecting to external stimuli. Herein, we designed a one-pot and in situ gelation system, which involved strategic selection of precursors for constructing IPN gels by simply mixing them. This design involved two types of gelation processes: RADA16 peptide self-assembling and covalent bond formation between chitosan (CH) and N-hydroxysuccinimide ester-terminated poly(ethylene glycol) (NHS–PEG–NHS). The gelation kinetics suggested that RADA16 peptide networks were formed independently, followed by the formation of CH/PEG networks in the mixture containing the three components. Culturing chondrocytes in CH/PEG/RADA16 demonstrated that construction of the IPN structure promoted the embedded chondrocyte properties for the formation of the articular cartilage. Moreover, lower inflammation and higher protein production were observed in mice implanted with CH/PEG/RADA16-containing chondrocytes than in those with clinically used atelocollagen gel, appealing the feasibility of the proposed IPN hydrogel design for use as cell culturing scaffolds in tissue regeneration.
DOI	10.1021/acs.chemmater.9b04725