オオサワ　シゲヒト   Oosawa Shigehito   大澤　重仁    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Enhanced function of chondrocytes in a chitosan‐based hydrogel to regenerate cartilage tissues by accelerating degradability of the hydrogel via a hydrolysable crosslinker
掲載誌名	正式名：Journal of applied polymer science. ISSNコード：00218995/10974628
掲載区分	国外
巻・号・頁	137(19),pp.1-8
総ページ数	8
著者・共著者	Shohei Ishikawa, Kazutoshi Iijima, Daisuke Matsukuma, Michihiro Iijima, Shigehito Osawa, Hidenori Otsuka
担当区分	責任著者
発行年月	2020/05/15
概要	Chitosan-based hydrogels as scaffolds for culturing chondrocytes were prepared using linkers with and without hydrolysable poly(DL-lactide) (PLA) segments. The evaluation of the cultured chondrocytes in them indicated that the accelerated degradation of the hydrogel via hydrolysis of the PLA slightly promoted production of the sulfated glycosaminoglycan and drastically improved that of collagen from the encapsulated chondrocytes, which are the chondrospecific extracellular matrix components. Furthermore, the accelerated degradability significantly upregulated the gene expression for Collagen II production and downregulated that for Collagen I production of the encapsulated chondrocytes. Because major component of the articular cartilage tissue is Collagen II-rich hyaline cartilage, these results suggest the degradation of the scaffolds is an important parameter in cartilage tissue regeneration and the accelerated degradability may have benefits on promotion of cartilage tissue regeneration especially from the viewpoint of hyaline cartilage-like collagen production.
DOI	10.1002/app.48893