オオサワ　シゲヒト   Oosawa Shigehito   大澤　重仁    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Direct Observation of Cell Surface Sialylation by Atomic Force Microscopy Employing Boronic Acid–Sialic Acid Reversible Interaction
掲載誌名	正式名：Analytical chemistry 略　 称：Anal Chem ISSNコード：00032700/15206882
掲載区分	国外
巻・号・頁	92(17),pp.11714-11720
著者・共著者	Shigehito Osawa, Akira Matsumoto, Yukie Maejima, Toshihiro Suzuki, Yuji Miyahara, Hidenori Otsuka
担当区分	筆頭著者
発行年月	2020/07/29
概要	Tracing cell surface sialylation dynamics at a scale of the glycolipoprotein microdomain (lipid rafts) formations remains an intriguing challenge of cellular biology. Here, we demonstrate that this goal is accessible, taking advantage of a boronic acid (BA)-based reversible molecular recognition chemistry. A BA-end-functionalized poly(ethylene glycol) was decorated onto an atomic force microscopy (AFM) cantilever, which provided a dynamic and sialic acid (SA)-specific imaging mode. Using this technique, we were able to heat map the SA expression levels not only on protein-decorated substrates but also directly on the cell surfaces, with a submicrometer scale resolution that may be relevant to that of the lipid rafts formation. The SA specificity and the binding reversibility of the probe were confirmed from its pH-dependent characteristics and an inhibition assay using free state SA. This finding may provide a noninvasive means for assessing a variety of SA-involved glycosylation dynamics spanning from physiology to pathology.
DOI	https://doi.org/10.1021/acs.analchem.0c01705