オオサワ　シゲヒト   Oosawa Shigehito   大澤　重仁    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode
掲載誌名	正式名：Bioconjugate chemistry 略　 称：Bioconjug Chem ISSNコード：10431802/15204812
掲載区分	国外
巻・号・頁	32(2),pp.239-244
総ページ数	5
著者・共著者	Akira Matsumoto, Shigehito Osawa, Takahiro Arai, Yukie Maejima, Hidenori Otsuka, Yuji Miyahara
担当区分	2nd著者
発行年月	2021/01/22
概要	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation. Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states. A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was “grafted-to” a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide–alkyne cycloaddition reaction. Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen. Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
DOI	10.1021/acs.bioconjchem.0c00657