セキネ　ヒデカズ   Sekine Hidekazu   関根　秀一    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	External pressure dynamics promote kidney viability and perfusate filtration during ex vivo kidney perfusion
掲載誌名	正式名：Scientific Reports 略　 称：Sci Rep ISSNコード：2045-2322
掲載区分	国外
出版社	Spuringer Nature
巻・号・頁	12(1),pp.21564
著者・共著者	HIGASHI Yuhei†, HOMMA Jun, SEKINE Hidekazu*, UAGO Hiroki, KOBAYASHI Eiji, SHIMIZU Tatsuya
担当区分	責任著者
発行年月	2022/12/13
概要	Normothermic machine perfusion (NMP) has not yet been established as a technique for preserving organs for a day. A key contributing factor to the same is that the perfusing solutions cannot circulate continuously and evenly in the organs. Here, we conceived a method of applying intermittent air pressure from outside the organ to assist its circulatory distribution during perfusion. We used a perfusion culture system while applying external pressure to culture rat kidneys and compared the circulatory distribution in the kidneys, changes in tissue morphology due to injury, and perfusate filtration. The intermittent pressurization (IMP) (-) group showed markedly poorer circulation on the upper side compared with that in the lower side, alongside histological damage. On the other hand, the IMP (+) group showed improved circulation in the upper side and had lesser histological damage. Furthermore, the IMP (+) group maintained the ability to filter perfusate for 24 h. In transplantation medicine and regenerative medicine research, this method has the potential to contribute to more efficient organ preservation and more functional tissue regeneration in the future.
DOI	10.1038/s41598-022-26147-5