マスダ　ガク   Masuda Gaku   益田　岳    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Detection of the Plasmodium falciparum Kelch-13 gene P553L mutation in sporozoites isolated from mosquito salivary glands in South-Central Vietnam.
掲載誌名	正式名：Parasites & vectors 略　 称：Parasit Vectors ISSNコード：17563305/17563305
掲載区分	国外
巻・号・頁	10(1),pp.308
著者・共著者	Maeno Yoshimasa, Quang Nguyen Tuyen, Culleton Richard, Kawai Satoru, Masuda Gaku, Hori Kaoru, Nakazawa Shusuke, Marchand Ron P
発行年月	2017/06
概要	BACKGROUND:Plasmodium falciparum has developed resistance against artemisinin in Southeast Asia. Mutations in the P. falciparum Kelch-13 (Pfk13) gene are associated with artemisinin resistance in vitro and in vivo. We investigated the prevalence of mutations in PfK13 from sporozoite-stage parasites isolated from the salivary glands of Anopheles dirus mosquitoes.METHODS:Mosquitoes were caught by human-landing catches at two locations within the Khanh Phu commune, South-Central Vietnam. Identification of Anopheles species was performed based on morphological features and nucleotide sequence analysis. Sporozoite-infected salivary glands were stored on filter paper and at 4-6 °C. A nested-PCR targeting the small subunit ribosomal RNA gene was used for Plasmodium species identification. Pfk13 was amplified by nested PCR, and subjected to nucleotide sequencing.RESULTS:Five of 33 P. falciparum sporozoite samples carried the P553L mutation at the PfK13 locus. This mutation has been recorded previously in Vietnam, but not in Khanh Hoa province, were surveys of K13 polymorphism have not previously been carried out.CONCLUSION:These results demonstrate the utility of mosquito-stage malaria parasite samples for studies on the molecular epidemiology of drug resistance.
DOI	10.1186/s13071-017-2247-9
PMID	28646896