オオツキ　ミチオ   Ootsuki Michio   大月　道夫    所属   医学部 医学科（東京女子医科大学病院）    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Expression of activating transcription factor 2 in inflammatory macrophages in obese adipose tissue
掲載誌名	正式名：Obesity 略　 称：Obesity (Silver Spring) ISSNコード：1930739X (Electronic)19307381 (Linking)
掲載区分	国外
巻・号・頁	21(4),pp.731-736
著者・共著者	Miyata, Y. Fukuhara, A. Otsuki, M. Shimomura, I.
発行年月	2013
概要	OBJECTIVE: White adipose tissue (WAT) of obesity is in the state of inflammation with progressive infiltration by macrophages and overproduction of reactive oxygen species (ROS), which can induce WAT dysfunction, including insulin resistance and adipocytokine dysregulation. Activating transcription factor 2 (ATF2) is a member of the ATF/cAMP response element binding family of transcription factors and known to be activated by cellular stressors, such as inflammatory cytokines, lipopolysaccharide (LPS), and ROS. DESIGN AND METHODS, RESULTS: Here, we show that ATF2 protein was significantly more induced in WAT of ob/ob mice compared with C57BL/6J mice. Total and phosphorylated ATF2 were highly expressed in infiltrated macrophages. Furthermore, flow cytometry analysis demonstrated that ATF2 expression was high in CD11c-positive/CD301-negative M1 macrophages. Phosphorylation of ATF2 was induced by treatment with either H2 O2 or LPS in RAW264.7 macrophage cells, and suppression of ATF2 expression by small-interfering RNA induced mRNA levels of ATF3, an anti-inflammatory molecule in macrophages in WAT. CONCLUSIONS: These results suggest that ATF2 is an important transcriptional factor relating to inflammation through the suppression of ATF3 in M1 macrophages of WAT.
DOI	10.1002/oby.20274
文献番号	23712976